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Intracellular Energy-Transfer Networks and High-Resolution Respirometry: A Convenient Approach for Studying Their
Marju Puurand1, Kersti Tepp2, Aleksandr Klepinin3
1Laboratory of Chemical Biology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. marju.puurand@kbfi.ee.
International Journal of Molecular Sciences
|September 29, 2018
Summary
This study introduces a method to measure cellular energy transfer networks. High-resolution respirometry and permeabilization reveal functional coupling between creatine kinase (CK) and adenylate kinase (AK) pathways and oxidative phosphorylation (OXPHOS).
Area of Science:
- Cellular biology
- Bioenergetics
- Biochemistry
Background:
- Cellular energy is compartmentalized using high-energy phosphate carriers for efficient use.
- Creatine kinase (CK) and adenylate kinase (AK) energy-transfer networks, coupled to oxidative phosphorylation (OXPHOS), regulate cellular energy fluxes.
- Understanding these networks is crucial for cellular function in health and disease.
Purpose of the Study:
- To present a method for studying the functional coupling between energy-transfer networks (CK, AK) and OXPHOS.
- To enable quantification of energy transfer dynamics in various cell and tissue types.
- To investigate the role of hexokinase in mitochondrial energy metabolism.
Main Methods:
- Selective permeabilization of the cellular outer membrane.
- High-resolution respirometry.
- Monitoring the effects of creatine, adenosine monophosphate, and glucose on cellular respiration.
Main Results:
- Demonstrated the ability to observe and quantify the stimulation of OXPHOS by creatine (via CK) and adenosine monophosphate (via AK).
- Showcased the investigation of functional coupling between hexokinase and mitochondria by measuring glucose's effect on respiration.
- Validated the approach for studying energy-transfer networks in small cell and tissue samples.
Conclusions:
- High-resolution respirometry combined with permeabilization provides a convenient and powerful approach to study cellular energy-transfer networks.
- This method facilitates the investigation of energy metabolism in both healthy and pathological conditions.
- The findings offer insights into cellular energy regulation and potential therapeutic targets.
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