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Artifactual phosphate binding due to impurities in [32P]orthophosphate
Analytical Biochemistry
|October 1, 1986
Abstract:
Many commercial preparations of [32P]orthophosphate contain radioactive impurities that interfere with binding and transport studies in biological systems. One type of impurity is micro-particulate whereas another may be pyrophosphate. Methods of removing these impurities from radiolabeled orthophosphate solutions are described.