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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Related Experiment Video

Updated: Feb 4, 2026

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development
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Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development

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Transcriptomics and single-cell RNA-sequencing.

Daniel C Chambers1,2, Alan M Carew3, Samuel W Lukowski4,5

  • 1Queensland Lung Transplant Program, The Prince Charles Hospital, Brisbane, QLD, Australia.

Respirology (Carlton, Vic.)
|September 29, 2018
PubMed
Summary
This summary is machine-generated.

This review covers transcriptomics and RNA-sequencing for analyzing cellular phenotypes. These molecular biology techniques offer detailed insights into gene expression for research and potential clinical applications.

Keywords:
pulmonary diseaseribonucleic acid sequencingsingle-cell analysistranscriptome

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Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics
  • Next-Generation Sequencing

Background:

  • Molecular biology advances over four decades enable detailed genome and transcriptome characterization.
  • Transcriptomics and next-generation sequencing provide high-resolution understanding of cellular phenotypes.
  • Gene expression analysis in specific physiological and pathological states is now possible.

Purpose of the Study:

  • To provide an overview of transcriptomics and RNA-sequencing for analyzing whole tissue and single cells.
  • To describe techniques and challenges in single-cell isolation and sequencing.
  • To explore the benefits of single-cell transcriptomics and its applications in pulmonary research and clinical practice.

Main Methods:

  • Review of transcriptomics techniques.
  • Discussion of RNA-sequencing methodologies for whole tissue and single cells.
  • Analysis of single-cell isolation and sequencing protocols.

Main Results:

  • Transcriptomics and RNA-sequencing offer unprecedented detail in understanding cellular phenotypes.
  • Single-cell analysis provides additional benefits and insights beyond whole tissue analysis.
  • These technologies are increasingly applied in pulmonary research.

Conclusions:

  • Transcriptomics and RNA-sequencing are powerful tools for molecular biology research.
  • Single-cell RNA sequencing presents unique advantages and challenges.
  • Future translation into clinical practice, particularly in pulmonary medicine, is anticipated.