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Updated: Feb 4, 2026
12:11
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
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Versatile nano-platform for tailored immuno-magnetic carriers.
Emanuela Bonaiuto1, Massimiliano Magro1,2, Luca Fasolato1
1Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell'Università 16, 35020, Legnaro, PD, Italy.
Analytical and Bioanalytical Chemistry
|September 30, 2018
Summary
Surface active maghemite nanoparticles (SAMNs) offer a versatile platform for creating custom immuno-magnetic nano-carriers. These tailored nano-carriers show promise for detecting specific foodborne pathogens like Campylobacter and Listeria.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Immunotechnology
Background:
- Custom immuno-magnetic devices are crucial for biomedical and biotechnological applications.
- Developing versatile platforms for tailored immuno-magnetic nano-carriers is an ongoing challenge.
- Surface active maghemite nanoparticles (SAMNs) present a promising solution.
Purpose of the Study:
- To propose SAMNs as a versatile platform for developing customized immuno-magnetic nano-carriers.
- To demonstrate the conjugation of antibodies for pathogen detection.
- To showcase applications in foodborne pathogen recognition.
Main Methods:
- Synthesis of surface active maghemite nanoparticles (SAMNs).
- Functionalization of SAMNs with rhodamine B isothiocyanate (RITC) and anti-Campylobacter fetus antibodies.
- Modification of SAMNs with avidin for biotinylated anti-Listeria monocytogenes antibody immobilization.
- Integration of immuno-magnetic carriers into PCR and piezoelectric quartz crystal microbalance (QCM) sensors.
Main Results:
- Successful conjugation of native and biotinylated antibodies onto SAMNs.
- Selective capture of Campylobacter fetus in the presence of other species, verified by PCR.
- Detection of Listeria monocytogenes in milk with a detection limit of 3 bacterial cells using a QCM sensor.
- Demonstrated versatility of SAMNs for specific pathogen recognition.
Conclusions:
- SAMNs provide a versatile and customizable platform for creating immuno-magnetic nano-carriers.
- These nano-carriers offer effective solutions for specific foodborne pathogen detection.
- The developed immuno-magnetic nano-carriers represent a competitive option for various applications.
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