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Updated: Jan 7, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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[Ecology and function of human microbiomes.]

Masahira Hattori1

  • 1Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.

Clinical Calcium
|October 1, 2018
PubMed
Summary
This summary is machine-generated.

The human body hosts trillions of bacteria forming site-specific microbiomes. Advanced sequencing technologies reveal the gut microbiome

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Area of Science:

  • Microbiology
  • Genomics
  • Human Health

Background:

  • The human body harbors trillions of bacteria, forming complex microbial communities known as microbiomes.
  • These microbiomes are site-specific, with distinct bacterial species colonizing different areas of the body.

Purpose of the Study:

  • To highlight the significant advancements in human microbiome research.
  • To emphasize the role of next-generation sequencing in understanding microbial communities.

Main Methods:

  • Development of culture-independent metagenomic analysis.
  • Application of next-generation sequencing technologies.

Main Results:

  • Human microbiome research has advanced significantly in the past decade.
  • Metagenomic analysis has enabled detailed study of microbial communities.
  • The human gut microbiome is profoundly linked to host health and disease.

Conclusions:

  • The gut microbiome plays a critical role in human health and disease.
  • Continued research using advanced sequencing is crucial for understanding host-microbe interactions.