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Updated: Feb 4, 2026

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
Published on: August 28, 2017
An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible
Ana M Ferreira1, Helena Passos1, Akiyoshi Okafuji2
1CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, 3810-193 Aveiro, Portugal.
Abstract:
Thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are here disclosed to act as integrated bioreaction-separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recover and reuse of the enzyme, along with the ZI-rich phase, contributing towards the development of sustainable biocatalytic processes.
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