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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Structure assisted impurity profiling for rapid method development in liquid chromatography.

Sebastian Schmidtsdorff1, Alexander H Schmidt2, Maria Kristina Parr3

  • 1Chromicent GmbH, Johann-Hittorf-Str. 8, 12489, Berlin, Germany; Freie Universität Berlin, Königin-Luise-Str. 2+4, 14195, Berlin, Germany.

Journal of Chromatography. A
|October 3, 2018
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Summary
This summary is machine-generated.

This study introduces a logD value calculation method to predict pH effects in chromatography, reducing screening time and improving analytical method development. The approach enhances robustness and efficiency for pharmaceutical analysis.

Keywords:
Chromatographic modelingDistribution coefficient (logD)Extended knowledge spaceHigh-performance liquid chromatography (HPLC)Method developmentQuality-by-design (QbD)

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Trial-and-error dominates analytical method development, leading to limited knowledge of method robustness and parameter influence.
  • This often results in practical challenges for analysts during routine use.

Purpose of the Study:

  • To present a pH-dependent logD value calculation for simulating pH effects in reverse-phase chromatography.
  • To demonstrate the reduction of in silico method screening time and enhance understanding of separation mechanics.
  • To develop a superior, robust analytical method for carbamazepine purity analysis.

Main Methods:

  • pH-dependent logD value calculation for in silico simulation.
  • Application of the logD approach to model substances for method screening.
  • Development and validation of a new carbamazepine purity analysis method.

Main Results:

  • Significant reduction in method screening time was achieved using in silico simulations.
  • Enhanced understanding of separation mechanisms was generated.
  • A novel carbamazepine purity analysis method was developed, outperforming the official monograph and demonstrating high robustness.

Conclusions:

  • The logD-based approach facilitates efficient, in silico-driven analytical method development.
  • This method enables the application of Quality-by-Design principles, meeting stringent pharmaceutical industry regulatory demands.