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Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the
Martin Černý1, Miroslav Berka2, Hana Habánová2
1Faculty of AgriSciences, Department of Molecular Biology and Radiobiology, CEITEC - Central European Institute of Technology, Phytophthora Research Centre, Mendel University in Brno, Brno, Czech Republic. martincerny83@gmail.com.
Abstract:
Peptide spectral libraries enable targeted identification and quantitation of low-abundance proteins in a complex plant proteome. Here we describe parallel protein and peptide fractionation techniques to improve plant proteome coverage and facilitate construction of spectral libraries.
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