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Related Concept Videos

Proteomics01:33

Proteomics

9.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Targeted Cancer Therapies02:57

Targeted Cancer Therapies

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The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
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Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

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Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
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Target Cell Response to Hormones01:22

Target Cell Response to Hormones

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Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
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DNA-only Transposons02:57

DNA-only Transposons

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

19.3K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Updated: Feb 4, 2026

Skeletal Muscle Gender Dimorphism from Proteomics
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Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

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Targeted Proteomics.

Yun Chen1, Liang Liu2

  • 1School of Pharmacy, Nanjing Medical University, Nanjing, China. ychen@njmu.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2018
PubMed
Summary
This summary is machine-generated.

Targeted proteomics uses selected reaction monitoring (SRM) assays with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to accurately quantify specific proteins. This method is detailed for analyzing proteins in both cellular and tissue samples.

Keywords:
Cells and tissue samplesLiquid chromatography-tandem mass spectrometryProtein quantificationTargeted proteomics

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Skeletal Muscle Gender Dimorphism from Proteomics
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Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • Targeted proteomics enables sensitive and reproducible quantification of specific proteins.
  • Selected reaction monitoring (SRM) assays are crucial for precise protein measurement.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a standard technique in proteomics.

Purpose of the Study:

  • To detail the quantitative analysis of target proteins.
  • To describe the application of targeted proteomics in biological samples.
  • To provide a reproducible methodology for protein quantification.

Main Methods:

  • Generating surrogate peptides from target proteins via proteolytic digestion.
  • Developing selected reaction monitoring (SRM) assays for peptide quantification.
  • Utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for high-throughput analysis.

Main Results:

  • Demonstrated high sensitivity and quantitative accuracy in protein detection.
  • Achieved reproducible results in both cell and tissue sample analyses.
  • Validated the targeted proteomics approach for specific protein quantification.

Conclusions:

  • Targeted proteomics is a powerful tool for precise protein quantification.
  • The described methodology is effective for analyzing proteins in complex biological matrices.
  • This approach facilitates advancements in biomarker discovery and disease research.