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Two-Dimensional Biochemical Purification for Global Proteomic Analysis of Macromolecular Protein Complexes

Reza Pourhaghighi1, Andrew Emili2,3,4,5

  • 1Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2018
PubMed

Keywords:
Biochemical separationFractionationHigh-performance liquid chromatography (HPLC)Ion exchange chromatographyIsoelectric focusingNanoflow liquid chromatography tandem mass spectrometry (nLC-MS/MS)Protein complexesProtein–protein interaction

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