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Comparison of sampling methods for profiling cervicovaginal microbiome in rhesus macaques
Brian A Schmidt1, Ronald Phillips1, Matthew Rolston2
1Center for Comparative Medicine, University of California, Davis, California.
Journal of Medical Primatology
|October 3, 2018
Summary
Cervicovaginal bacteria increase HIV risk by causing inflammation. Profiling the vaginal microbiome is key for vaccine development, and cervicovaginal lavage offers a viable sampling method.
Area of Science:
- Microbiome research
- Immunology
- Vaccinology
Background:
- Cervicovaginal bacteria contribute to inflammation, elevating HIV susceptibility.
- Understanding the cervicovaginal microbiome is crucial for developing effective HIV vaccines.
Purpose of the Study:
- To assess the comparability of microbiome profiles obtained via cervicovaginal lavage versus vaginal swabs.
- To evaluate cervicovaginal lavage as a potential sampling strategy for non-human primate (NHP) vaccine studies.
Main Methods:
- Microbiome profiling of cervicovaginal samples.
- Comparison of sample types: cervicovaginal lavage and vaginal swabs.
Main Results:
- Microbiome profiles from cervicovaginal lavage are comparable to those from vaginal swabs.
- Vaginal microbiome composition can be reliably assessed using lavage.
Conclusions:
- Cervicovaginal lavage is a suitable method for capturing the vaginal microbiome profile.
- Lavage may be a practical sampling strategy for future NHP HIV vaccine research.
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