Related Experiment Video

Updated: Feb 4, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.5K

MiR-125b suppresses the carcinogenesis of osteosarcoma cells via the MAPK-STAT3 pathway

Tao Xiao1, You Zhou1, Hui Li1

  • 1Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, China.

Journal of Cellular Biochemistry
|October 3, 2018
PubMed

Insights

MicroRNA miR-125b suppresses osteosarcoma (OS) growth by targeting MKK7, which regulates STAT3. This miR-125b-MKK7-STAT3 pathway is crucial in OS development and offers a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNA miR-125b is frequently dysregulated in various cancers, including osteosarcoma (OS).
  • The precise role of miR-125b in OS initiation and progression remains unclear.
  • Signal transducer and activator of transcription 3 (STAT3) is implicated in cancer development.

Purpose of the Study:

  • To elucidate the function of miR-125b in osteosarcoma.
  • To investigate the molecular mechanisms by which miR-125b influences OS progression.
  • To identify potential therapeutic targets for OS treatment.

Main Methods:

  • In vitro assays to assess cell proliferation and invasion.
  • In vivo studies to evaluate tumor formation.
  • Western blot analysis to examine protein expression and phosphorylation.
  • Analysis of MKK7 expression in OS tissues and correlation with prognosis.

Main Results:

  • miR-125b directly suppresses MAP kinase kinase 7 (MKK7) expression in OS cells.
  • MKK7 dephosphorylates and inactivates STAT3.
  • Overexpression of MKK7 in OS correlates with poor prognosis.
  • miR-125b inhibits OS cell proliferation, invasion, and tumor growth by downregulating MKK7.
  • MKK7 influences STAT3 activation at an epigenetic level.

Conclusions:

  • The miR-125b-MKK7-STAT3 axis is a key pathway in osteosarcoma oncogenesis.
  • miR-125b acts as a tumor suppressor in OS by targeting MKK7.
  • This pathway represents a promising therapeutic target for osteosarcoma treatment.
Keywords:
MAP2K7microRNA-125bosteosarcomasignal transducer and activator of transcription 3

More Related Videos

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System08:54

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

10.3K
Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells08:52

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Published on: June 13, 2018

9.3K

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
8.4K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.2K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
914
Auditory Pathway01:15

Auditory Pathway

Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking...
7.4K
ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
4.8K
Respiration Pathways01:26

Respiration Pathways

Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
784