Phosphodiesters quaternary ammonium nanoparticles as label-free light scattering probe for turn-off detection of tyrosine

In this contribution, a new highly sensitive and selective sensor of the determination of tyrosine has been proposed based on the downturn effect of light scattering (LS) using phosphodiesters quaternary ammonium nanoparticles (PQANPs). Phosphodiesters quaternary ammonium (PQA), one of Gemini zwitterionic surfactants, self-aggregated into the micelle named as PQANPs, which generated strong LS signal in aqueous solution under the optimum condition. Interestingly, the powerful LS intensity of PQANPs with the maximum peak located at 391 nm significantly decreased after introducing trace amount of tyrosine. The decreased value of the LS intensity of the PQA-tyrosine system (ΔI_LS) was in proportion to tyrosine concentration in the ranges from 5.5 × 10^-8 mol/L to 4.68 × 10^-6 mol/L, with a detection limit of 1.38 × 10^-8 mol/L. Based on this decreased LS situation, the novel approach of the determination of tyrosine was first developed. The reaction mechanism for the interaction between PQANPs and tyrosine was also investigated. Moreover, the proposed LS assay was applied to the detection of tyrosine concentration in human serum and urine samples with satisfactory results.