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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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Cryo scanning transmission x-ray microscope optimized for spectrotomography.

A F G Leontowich1, R Berg1, C N Regier1

  • 1Canadian Light Source, Inc., Saskatoon, Saskatchewan S7N 2V3, Canada.

The Review of Scientific Instruments
|October 4, 2018
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Summary

A new cryo scanning transmission X-ray microscope (cryo-STXM) offers advanced capabilities for analyzing frozen samples at the Canadian Light Source. This innovative instrument provides high-resolution 3D and 4D chemical mapping for materials science research.

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Area of Science:

  • Materials Science
  • Synchrotron Radiation
  • Microscopy

Background:

  • Soft X-ray microscopy is crucial for analyzing delicate biological and materials samples.
  • Existing techniques face limitations in resolution, radiation damage, and contamination control.
  • Advancements in instrumentation are needed to improve data acquisition and analytical capabilities.

Purpose of the Study:

  • To design, commission, and demonstrate the capabilities of a new cryo scanning transmission X-ray microscope (cryo-STXM).
  • To achieve high spatial and spectral resolution for analyzing frozen samples.
  • To enable advanced imaging techniques like cryo-spectrotomography.

Main Methods:

  • The cryo-STXM was designed and commissioned at the Canadian Light Source synchrotron.
  • The instrument operates in the soft X-ray region (100–4000 eV).
  • Samples are loaded via a load lock and can be rotated ±70° for tomography, maintained at 92 K and 10-9 Torr.

Main Results:

  • Achieved spatial resolution of 30 nm and spectral resolution of 0.1 eV.
  • Demonstrated significantly improved data acquisition efficiency for imaging and tomography.
  • Successfully produced 2D images, 3D tomograms, and 4D chemical maps of automotive hydrogen fuel cell thin sections.

Conclusions:

  • The newly commissioned cryo-STXM is a powerful tool for high-resolution, low-damage analysis of frozen samples.
  • The instrument enables advanced cryo-spectrotomography in the soft X-ray region.
  • Its performance and capabilities open new avenues for materials science research, particularly in energy applications.