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Updated: Feb 4, 2026

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
Published on: January 1, 2017
Glycerol induces early fibrosis in regenerating rat skeletal muscle
Mohamed A A Mahdy1,2,3, Katsuhiko Warita1,4, Yoshinao Z Hosaka1,4
1Laboratory of Basic Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8515, Japan.
Abstract:
Glycerol has been recently used to induce muscle adiposity in mice. However, its effects on the rat muscles have not been investigated previously. Therefore, we investigated the regeneration outcomes of rat muscles following glycerol-induced injury at different time points. Glycerol injection induced myofiber degeneration with extensive inflammatory infiltration on day 4 followed by appearance of regenerating myotubes on day 7 after injury without adipocyte infiltration. Meanwhile, a significant collagen deposition at early stage of regeneration that increased together with persistent inflammatory infiltration up to day 14 after injury indicates impaired regeneration. In conclusion, glycerol injury in rats is more suitable as a fibrosis-inducing model than in mice due to earlier and higher accumulation of fibrous tissue with lacking adipogenesis.
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