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Flow cytometry for pediatric platelets

Anastasia A Ignatova1, Evgeniya A Ponomarenko1,2, Dmitry M Polokhov1

  • 1a Cellular Hemostasis and Thrombosis Lab , National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, Russian Ministry of Healthcare , Moscow , Russian Federation.

Platelets
|October 5, 2018
PubMed

Insights

A new flow cytometry method simplifies comprehensive platelet function testing for both children and adults. This screening tool aids in diagnosing platelet disorders and assessing hemostatic function across various clinical conditions.

Area of Science:

  • Hematology
  • Immunology
  • Clinical Diagnostics

Background:

  • Platelet hemostatic function is crucial but can be impaired in numerous inherited and acquired conditions, affecting both adults and children.
  • Assessing platelet function is vital for research and clinical practice, especially in pediatric patients where blood volume and collection methods pose challenges.
  • Existing methods for platelet function analysis can be complex, necessitating simpler, comprehensive screening tools.

Purpose of the Study:

  • To develop and validate a straightforward, flow cytometry-based screening method for comprehensive whole blood platelet function testing.
  • To adapt and validate this method for both pediatric and adult patient samples within a hospital setting.
  • To analyze parameter distribution in healthy adult and pediatric donor populations.

Main Methods:

  • Utilized a flow cytometry-based approach for whole blood platelet function analysis.
  • Assessed multiple platelet parameters including scatter, glycoprotein levels (Ib, IIb-IIIa), active integrins (PAC-1 binding), alpha-granule release (P-selectin), dense granule function (mepacrine uptake/release), and procoagulant activity (annexin V binding).
  • Tested both resting and dual-agonist-stimulated platelets, with detailed discussion of preanalytical and analytical variables.

Main Results:

  • Successfully validated a simple flow cytometry screening method for comprehensive platelet function testing in pediatric and adult samples.
  • Demonstrated the method's utility across a spectrum of conditions, including inherited platelet disorders, immune thrombocytopenia, and effects of therapy.
  • Analyzed and provided parameter distributions for healthy adult and pediatric donor populations.

Conclusions:

  • The described flow cytometry method offers a simple, comprehensive, and validated approach for assessing platelet function in diverse clinical scenarios.
  • This screening tool is particularly valuable for pediatric hematology, addressing limitations in blood volume and collection methods.
  • The method provides critical data for diagnosing platelet disorders and monitoring hemostatic function in both research and clinical settings.

Keywords:
Flow cytometryimmune thrombocytopeniapediatric plateletsplatelet functionthrombocytopathy

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