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Differential requirement for accessory cells in polyclonal T-cell activation.

M L Sopori, Y L Hurt, S Cherian

    Cellular Immunology
    |March 1, 1987
    PubMed
    Summary

    Accessory cells (AC) are crucial for T-cell activation, with specific requirements for Ia-positive or Ia-negative cells depending on the stimulus. Macrophages and T-cell accessory cells (TAPC) demonstrate distinct roles in T-cell proliferation and receptor expression.

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    Area of Science:

    • Immunology
    • Cell Biology
    • T-cell Activation

    Background:

    • Resting T cells require accessory cells (AC) and/or soluble factors for activation by various stimuli.
    • The role of Ia-positive (OX-6+) and Ia-negative (OX-6-) T cells as potential ACs needs further investigation.
    • Understanding T-cell-AC interactions is vital for comprehending immune responses.

    Purpose of the Study:

    • To investigate the requirement for accessory cells (AC) and soluble factors in activating resting T cells.
    • To differentiate the roles of Ia-positive and Ia-negative ACs in T-cell responses to mitogens and antigens.
    • To characterize the accessory function of T-cell accessory cells (TAPC) and their radiosensitivity.

    Main Methods:

    • Utilized highly purified rat Ia-negative (OX-6-) and Ia-positive (OX-6+) T cells.

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  • Employed various cells as ACs, including macrophages (M phi), OX-6+ T cells, and fibroblast cell lines.
  • Assessed T-cell proliferation and interleukin-2 receptor (IL-2R) expression in response to Con A, PHA, sodium periodate, and antigen.

    • Main Results:

    • An obligatory requirement for ACs was observed for OX-6- T cell proliferation and IL-2R expression in response to Con A, PHA, and antigen, not overcome by IL-1/IL-2.
    • Antigen activation of OX-6- T cells required Ia+ ACs, while mitogen activation could utilize Ia- ACs.
    • Macrophages were efficient ACs, while TAPC function varied; TAPC's antigen-presenting ability was radiosensitive, but Con A/PHA support was radioresistant.

    Conclusions:

    • T-cell activation necessitates accessory cells, with specific cell types and Ia expression influencing the response.
    • The molecules involved in T-cell-AC interactions differ based on the AC source and the type of proliferative stimulus.
    • This study provides insights into the complex mechanisms governing T-cell activation and accessory cell function.