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Updated: Jul 30, 2026

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
Published on: January 31, 2014
Do collagen cross-linkers improve dentin's bonding receptiveness?
C Parise Gré1, D Pedrollo Lise2, A P Ayres3
1KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & UZ Leuven (University Hospitals Leuven), Dentistry, Kapucijnenvoer 7, B-3000, Leuven, Belgium; Federal University of Santa Catarina (UFSC), Department of Dentistry, Operative Dentistry, Campus Reitor João David Ferreira Lima, s/n - Trindade, Florianópolis, SC, 88040-900, Brazil.
Proanthocyanidin (PA) effectively maintained dentin-adhesive bond durability over 6 months. Glutaraldehyde (GA) and riboflavin (RB) did not improve bond stability, similar to water application.
Area of Science:
- Biomaterials Science
- Dental Adhesion
- Nanotechnology
Background:
- Dentin biomodification aims to enhance adhesive bond durability.
- Collagen cross-linkers are investigated for this purpose.
- Clinical efficacy of cross-linkers at realistic application times remains unclear.
Purpose of the Study:
- To evaluate the impact of three cross-linkers on dentin-adhesive bond durability.
- To assess the mini-interfacial fracture toughness (mini-iFT) after 6-month aging.
- To compare the effects of glutaraldehyde (GA), proanthocyanidin (PA), and riboflavin (RB) in etch&rinse (E&R) and self-etch (SE) bonding modes.
Main Methods:
- Sixty molars were treated with GA, PA, RB, or water (control) for 60s.
- Four adhesives were applied using E&R or SE modes.
- Mini-iFT was measured after 1 week and 6 months of aging.
- Statistical analysis used a linear model (p<0.05).
Main Results:
- Proanthocyanidin (PA) showed no significant decrease in mini-iFT (p<0.05).
- Glutaraldehyde (GA) and riboflavin (RB) resulted in decreased mini-iFT.
- The bond degradation with GA and RB was comparable to water application.
Conclusions:
- Proanthocyanidin (PA) is a promising cross-linker for maintaining stable dentin-adhesive interfaces.
- Glutaraldehyde (GA) and UVA-activated riboflavin (RB) did not enhance bond durability under clinical conditions.
- Dentin biomodification with PA offers potential for improved long-term dental restorations.
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