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BmNPV-miR-415 up-regulates the expression of TOR2 via Bmo-miR-5738

Xueliang Cao1,2, Yong Huang3,4, Dingguo Xia1,4

  • 1Jiangsu University of Science and Technology, Zhenjiang City 212018, Jiangsu Province, PR China.

Saudi Journal of Biological Sciences
|October 9, 2018
PubMed

Insights

A novel Bombyx mori nucleopolyhedrosis virus (BmNPV) miRNA, BmNPV-miR-415, up-regulates the expression of the protein target of rapamycin isoform 2 (TOR2). This occurs indirectly via Bmo-miR-5738, highlighting complex virus-host interactions.

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • MicroRNAs (miRNAs) are crucial in host-pathogen interactions.
  • Virus-encoded miRNAs play significant roles in viral pathogenesis.
  • A novel Bombyx mori nucleopolyhedrosis virus (BmNPV)-encoded miRNA, BmNPV-miR-415, was previously identified.

Purpose of the Study:

  • To investigate the function and targets of the BmNPV-encoded miRNA miR-415.
  • To elucidate the regulatory mechanism of BmNPV-miR-415 in Bombyx mori cells.
  • To identify host protein targets affected by BmNPV infection.

Main Methods:

  • Construction and transfection of a BmNPV-miR-415 expression vector into BmN cells.
  • Two-dimensional gel electrophoresis and mass spectrometry to identify differentially expressed proteins.
  • Analysis of protein target of rapamycin isoform 2 (TOR2) expression.

Main Results:

  • BmNPV-miR-415 was successfully expressed in BmN cells.
  • The protein target of rapamycin isoform 2 (TOR2) was identified as differentially expressed.
  • TOR2 is not a direct target but its expression is up-regulated by BmNPV-miR-415 through Bmo-miR-5738.

Conclusions:

  • BmNPV-miR-415 indirectly up-regulates TOR2 expression via Bmo-miR-5738.
  • Bmo-miR-5738 can be induced by BmNPV, suggesting a complex regulatory network.
  • This study reveals a novel mechanism of virus-mediated host gene regulation.
Keywords:
3′ UTRBmNPVBombyx moriMicroRNAsTOR2

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