Comprehensive analysis of aberrantly expressed profiles of messenger RNA in alcoholic liver disease
Jinhui Sun1, Baolong Li2, Antao Sun3
1Gastroenterology Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.
Background:
Alcoholic liver disease (ALD) is one of the major cause of morbidity and mortality of clinical liver disease worldwide. Until today, although many general therapies are carried out and several molecular targets have been proposed to act as the potential therapeutic targets, more accurate molecular targets and more effective therapeutic methods remain needed.
Material And Methods:
In the study, we analyze the differential expression genes (DEGs) between the patients with ALD and healthy controls. Gene Ontology enrichment and KEGG signaling pathway analysis are performed to identify the function of DEGs. Some significant molecules are proposed to act as the potential therapeutic targets for ALD. RNA data of 15 ALD tissues and 7 normal tissues for RNA expression analysis were obtained. DEGs in ALD samples compared with normal tissues identified through the limma R package and subjected to network analysis.
Results:
As a result, we obtained a total of 274 DEGs that mainly involved in biological processes related to the angiogenesis, stress reaction, synthesis, and metabolism of organic acids. Network analysis obtained several genes with high network degree and fold change. Some significant molecules are proposed to act as the potential therapeutic targets for ALD.
Conclusions:
Our research identified some new progression-related genes of alcohol liver diseases, which could be regarded as the new targets for the early diagnosis and therapeutic management in ALD.
Insights
This study identified novel genes linked to alcoholic liver disease (ALD) progression. These findings offer potential new targets for early diagnosis and effective treatment of ALD.
Area of Science:
- Hepatology
- Molecular Biology
- Genomics
Background:
- Alcoholic liver disease (ALD) is a significant global cause of liver morbidity and mortality.
- Current therapies for ALD are insufficient, highlighting the need for more precise molecular targets and effective treatments.
Purpose of the Study:
- To identify differentially expressed genes (DEGs) in ALD patients compared to healthy controls.
- To explore the functional roles of DEGs through Gene Ontology and KEGG pathway analysis.
- To propose significant molecules as potential therapeutic targets for ALD.
Main Methods:
- RNA sequencing data from 15 ALD tissues and 7 normal tissues were analyzed.
- Differential gene expression analysis was performed using the limma R package.
- Network analysis was conducted on identified DEGs.
Main Results:
- A total of 274 DEGs were identified in ALD samples.
- DEGs were primarily associated with angiogenesis, stress response, and organic acid metabolism.
- Network analysis revealed key genes with high network degree and fold change, suggesting potential therapeutic targets.
Conclusions:
- New genes associated with alcoholic liver disease progression were discovered.
- These identified genes represent potential novel targets for early diagnosis and therapeutic strategies in ALD.
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