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Updated: Feb 4, 2026

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
Published on: March 7, 2017
Continuous, label-free, 96-well-based determination of cell migration using confluence measurement
Christian Mayr1,2, Marlena Beyreis1,3, Heidemarie Dobias4
1a Institute of Physiology and Pathophysiology, Laboratory for Tumour Biology and Experimental Therapies (TREAT) , Paracelsus Medical University Salzburg , Salzburg , Austria.
Abstract:
Cellular migration is essential in diverse physiological and pathophysiological processes. Here, we present a protocol for quantitative analysis of migration using confluence detection allowing continuous, non-endpoint measurement with minimal hands-on time under cell incubator conditions. Applicability was tested using substances which enhance (EGF) or inhibit (cytochalasin D, ouabain) migration. Using a gap-closure assay we demonstrate that automated confluence detection monitors cellular migration in the 96-well microplate format. Quantification by % confluence, % cell free-area or % confluence in cell-free area against time, allows detailed analysis of cellular migration. The study describes a practicable approach for continuous, non-endpoint measurement of migration in 96-well microplates and for detailed data analysis, which allows for medium/high-throughput analysis of cellular migration in vitro.
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