Related Experiment Video
Updated: Feb 4, 2026
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
Published on: November 29, 2024
Quantification of phenolic acid metabolites in humans by LC-MS: a structural and targeted metabolomics approach
Mark E Obrenovich1,2,3,4, Curtis J Donskey5,6, Isaac T Schiefer4
1Pathology & Laboratory Medicine Service, Louis Stokes Cleveland Department of Veteran's Affairs Medical Center, Cleveland, OH, 44106, USA.
Aim:
Co-metabolism between a human host and the gastrointestinal microbiota generates many small phenolic molecules such as 3-hydroxy-3-(3-hydroxyphenyl)propanoic acid (3,3-HPHPA), which are reported to be elevated in schizophrenia and autism. Characterization of these chemicals, however, has been limited by analytic challenges.
Methodology/Results:
We applied HPLC to separate and quantify over 50 analytes, including multiple structural isomers of 3,3-HPHPA in human cerebrospinal fluid, serum and urine. Confirmation of identity was provided by NMR, by MS and other detection methods. The highly selective methods support rapid quantification of multiple metabolites and exhibit superior chromatographic behavior.
Conclusion:
An improved ultra-HPLC-MS/MS and structural approaches can accurately quantify 3,3-HPHPA and related analytes in human biological matrices.
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