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Updated: Feb 4, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures
Published on: January 13, 2021
Structured illumination approaches for super-resolution in plant cells
Sidney L Shaw1, David Thoms1, James Powers1
1Department of Biology, Light Microscopy Imaging Center, Indiana University, Bloomington, IN, USA.
Super-resolution microscopy offers new ways to see molecules in cells. This review discusses super-resolution imaging in plants, overcoming challenges like the cell wall for better molecular visualization.
Area of Science:
- Biological microscopy
- Cellular and molecular biology
- Plant science
Background:
- Super-resolution microscopy advances molecular and protein distribution studies in cells.
- Improved optical designs and fluorescence collection enhance signal from labels.
- Structured illumination extracts higher spatial frequencies from conventional probes.
Purpose of the Study:
- To review prominent super-resolution imaging methods for living plant specimens.
- To discuss challenges and future directions for super-resolution in plants.
Main Methods:
- Focuses on two prominent super-resolution techniques applicable to plant systems.
- Addresses challenges related to imaging through the plant cell wall.
Main Results:
- Super-resolution imaging in plants is hindered by optical aberrations from the cell wall.
- Despite challenges, advancements enable visualization of molecular distributions in plant cells.
Conclusions:
- Super-resolution microscopy holds significant potential for plant biology research.
- Overcoming imaging barriers is crucial for broader adoption and future advancements.
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