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Updated: Feb 4, 2026

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Published on: November 30, 2018
In Vivo Synthesis of Nanocomposites Using the Recombinant Escherichia coli
Jae Hwan Jung1, Sang Yup Lee2, Tae Seok Seo3
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Abstract:
Biogenic gold nanorod (AuNR)-Ag core-shell nanocomposites (NCs) are synthesized by using recombinant Escherichia coli to demonstrate in vivo synthesis of biogenic NCs for the first time. The chemically synthesized AuNRs are internalized into the E. coli, and Ag ions are reduced and grown on the surface of the AuNRs with the assistance of metal-binding proteins, producing biogenic core-shell AuNR-Ag NCs. The core-shell structure of the biogenic AuNR-Ag NC is confirmed by transmission electron microscopy and energy-dispersive X-ray analysis. The biogenic AuNR-Ag NCs exhibit good plasmonic effects. While the core-shell morphology of the AuNR and Ag NCs is due to the similar lattice of Au and Ag, the shape of the biogenic NCs composed of gold nanoparticles and Fe is aciniform, and that of Fe3 O4 NPs and Au/Ag is a network structure, demonstrating the controllability of biogenic nanosynthesis using diverse metal combinations with different NC morphologies.
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