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The long noncoding RNA XIST protects cardiomyocyte hypertrophy by targeting miR-330-3p

Yuewu Chen1, Xianxia Liu1, Lei Chen1

  • 1Department of Cardiovascular Medicine, The Second Affiliated Hospital of Hainan Medical University, Haikou, 570100, Hainan Province, China.

Biochemical and Biophysical Research Communications
|October 10, 2018
PubMed

Insights

Long non-coding RNA XIST and microRNA-330-3p regulate cardiac hypertrophy. XIST promotes S100B expression, preventing hypertrophy via the XIST/miR-330-3p/S100B pathway.

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Gene Regulation

Background:

  • Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play roles in cardiovascular diseases.
  • The specific function of lncRNA XIST in cardiac hypertrophy requires further investigation.

Purpose of the Study:

  • To elucidate the role of lncRNA XIST in cardiomyocyte hypertrophy.
  • To identify the molecular mechanism involving XIST, miR-330-3p, and S100B in cardiac hypertrophy.

Main Methods:

  • Quantitative real-time PCR to measure gene expression.
  • In vitro gain- and loss-of-function studies in cardiomyocytes.
  • Bioinformatic analysis and luciferase reporter assays to confirm target interactions.

Main Results:

  • XIST was upregulated in hypertrophic cardiac tissue and cardiomyocytes.
  • XIST inhibition exacerbated hypertrophy, while XIST overexpression attenuated it.
  • XIST and miR-330-3p exhibited reciprocal suppression.
  • XIST promoted S100B expression by sponging miR-330-3p, thereby preventing cardiac hypertrophy.

Conclusions:

  • A novel XIST/miR-330-3p/S100B pathway was identified that modulates cardiomyocyte hypertrophy.
  • This pathway represents a potential therapeutic target for cardiovascular diseases involving cardiac hypertrophy.
Keywords:
Cardiac hypertrophyCardiomyocyteLong noncoding RNAS100BXISTmiR-330-3p

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