Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Cells and Tissues02:01

Plant Cells and Tissues

65.7K
Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
65.7K
Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

28.5K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
28.5K
Plant Tissue Culture02:57

Plant Tissue Culture

40.7K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
40.7K
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

24.3K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.3K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

11.5K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.5K
Plant Cell Wall02:43

Plant Cell Wall

60.3K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
60.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Redox-responsive ionic covalent organic framework films for hydroxide ion transport and tunable mechanical properties.

Chemical science·2026
Same author

Topological Control of Dual Protonic-Electronic Conduction in Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same author

Covalent Organic Frameworks with Intrinsic Pendant Aldehydes for Efficient Nitrate Electroreduction.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Interface-Induced Concentration Enhancement in Glycine Solutions Investigated Using Surface Plasmon Resonance Spectroscopy and Molecular Dynamics Simulations.

The journal of physical chemistry letters·2026
Same author

Metabolic thermodynamics: pertinent reference state and energy potentials.

The FEBS journal·2026
Same author

Disrupted energy metabolism is associated with retinal ganglion cell degeneration in autosomal dominant optic atrophy.

Science advances·2026

Related Experiment Video

Updated: Feb 4, 2026

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
08:36

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants

Published on: September 16, 2022

2.3K

Highly Active Protein Surfaces Enabled by Plant-Based Polyphenol Coatings.

Ana M L Sousa1, Tai-De Li2, Sabu Varghese3

  • 1WestCHEM/Department of Pure & Applied Chemistry , University of Strathclyde , 295 Cathedral Street , Glasgow G1 1XL , U.K.

ACS Applied Materials & Interfaces
|October 10, 2018
PubMed
Summary

Cross-linked polyphenol coatings offer a versatile method for immobilizing active proteins on various materials. This approach enhances protein activity and broadens applications in sensing and biocatalysis compared to traditional methods.

Keywords:
biointerfaceenzyme biocatalysispolydopaminepolyphenolprotein immobilization

More Related Videos

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
10:18

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

Published on: November 1, 2016

21.9K
Adherence of Bacteria to Plant Surfaces Measured in the Laboratory
07:07

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Published on: June 19, 2018

13.3K

Related Experiment Videos

Last Updated: Feb 4, 2026

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
08:36

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants

Published on: September 16, 2022

2.3K
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
10:18

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

Published on: November 1, 2016

21.9K
Adherence of Bacteria to Plant Surfaces Measured in the Laboratory
07:07

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Published on: June 19, 2018

13.3K

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Biotechnology

Background:

  • Protein immobilization on material supports is crucial for applications in sensing, biocatalysis, and biomedical interfaces.
  • Developing efficient and versatile methods for protein functionalization remains an active area of research.

Purpose of the Study:

  • To demonstrate the advantages of aqueous-processed cross-linked polyphenol coatings for immobilizing active proteins on diverse materials.
  • To optimize protein immobilization for enhanced activity and broader material compatibility.

Main Methods:

  • Utilized aqueous-processed cross-linked polyphenol coatings for protein immobilization.
  • Employed enzyme assays, X-ray photoelectron spectroscopy, silver staining, contact angle, solid-state 13C NMR, HPLC, and ESI-MS for characterization.
  • Compared polyphenol coatings with polydopamine and other approaches.

Main Results:

  • Achieved significant active protein loading (e.g., 4.8 mg·m-2 active phosphatase on polyester fabric).
  • Demonstrated higher protein activity (over an order of magnitude in some cases) and broader pH/material compatibility with polyphenol coatings.
  • Showcased the functionality of coatings derived from plant polyphenol extracts, even at lower purity.

Conclusions:

  • Aqueous-processed cross-linked polyphenol coatings provide an effective and versatile platform for active protein immobilization.
  • Optimizing functionalization for protein activity, rather than coating thickness, yields superior results.
  • Polyphenol coatings offer advantages over polydopamine, particularly due to the absence of amine groups, leading to improved active biofunctional surfaces.