Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Silica containing primary hydroxyl groups for high-performance affinity chromatography.

K Ernst-Cabrera, M Wilchek

    Analytical Biochemistry
    |December 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The conversion of azo-quenchers to fluorophores.

    Analytical biochemistry·2019
    Same author

    Affinity purification of the avidin protein family, based on crystal structures of avidin-HABA complexes.

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2018
    Same author

    The avidin-biotin complex in immunology.

    Immunology today·2014
    Same author

    Editors' note.

    Applied biochemistry and biotechnology·2013
    Same author

    Fast mass spectrometry detection of tryptophan-containing peptides and proteins by reduction with pyridine-borane.

    Analytical biochemistry·2013
    Same author

    Avidin-biotin technology : preparation of biotinylated probes.

    Methods in molecular biology (Clifton, N.J.)·2012
    Same journal

    The Long Run: A Tribute to Arthur Joseph Lawrence Cooper.

    Analytical biochemistry·2026
    Same journal

    Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

    Analytical biochemistry·2026
    Same journal

    Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

    Analytical biochemistry·2026
    Same journal

    UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

    Analytical biochemistry·2026
    Same journal

    IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

    Analytical biochemistry·2026
    Same journal

    A PCR-based assay for distinguishing between 293, 293T, and 293E cell lines.

    Analytical biochemistry·2026
    See all related articles

    Researchers developed a new silica material with primary hydroxyl groups for efficient protein purification. This novel silica resin enables fast and high-yield purification of antibodies and enzymes using affinity chromatography.

    Area of Science:

    • Biomaterials Science
    • Surface Chemistry
    • Chromatography

    Background:

    • Silica-based materials are widely used in chromatography.
    • Functionalization of silica surfaces is crucial for developing advanced separation media.
    • Efficient methods for protein immobilization are needed for affinity purification.

    Purpose of the Study:

    • To synthesize a novel silica derivative functionalized with primary hydroxyl groups.
    • To develop an efficient method for protein coupling to the silica support.
    • To evaluate the performance of the new silica in affinity chromatography for protein purification.

    Main Methods:

    • A four-step reaction procedure was employed to introduce primary hydroxyl groups onto the silica surface.
    • Activation of hydroxyl groups using chloroformates or carbodiimidazole.

    Related Experiment Videos

  • Coupling of proteins (antibodies, trypsin) to the activated silica.
  • Affinity chromatography using the developed silica columns.

    • Main Results:

    • Successfully synthesized primary hydroxyl-containing silica.
    • Achieved high-yield protein coupling under mild conditions.
    • Demonstrated fast and efficient purification of antibodies and trypsin using affinity columns.
    • Investigated the effect of pressure on affinity complex dynamics.

    Conclusions:

    • The developed hydroxyl-functionalized silica is a versatile and effective support for affinity chromatography.
    • This method offers a robust approach for the preparation of protein purification media.
    • The new silica derivatives facilitate high-performance bioseparations.