Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.7K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.7K
Toughness and Hardness of Aggregate01:22

Toughness and Hardness of Aggregate

620
Toughness and hardness are critical properties of aggregate materials used in concrete, particularly on pavement surfaces and industrial flooring subjected to heavy loads. Toughness is defined as the aggregate's resistance to failure by impact and is measured by the aggregate impact value (AIV). For this, the aggregate impact value test is performed, wherein the impact is delivered by a standard hammer, which falls freely under its own weight onto the aggregates. The aggregates fragment in...
620
Random Error01:04

Random Error

9.8K
Random or indeterminate errors originate from various uncontrollable variables, such as variations in environmental conditions, instrument imperfections, or the inherent variability of the phenomena being measured. Usually, these errors cannot be predicted, estimated, or characterized because their direction and magnitude often vary in magnitude and direction even during consecutive measurements. As a result, they are difficult to eliminate. However, the aggregate effect of these errors can be...
9.8K
Random Variables01:09

Random Variables

17.8K
A random variable is a single numerical value that indicates the outcome of a procedure. The concept of random variables is fundamental to the probability theory and was introduced by a Russian mathematician, Pafnuty Chebyshev, in the mid-nineteenth century.
Uppercase letters such as X or Y denote a random variable. Lowercase letters like x or y denote the value of a random variable. If X is a random variable, then X is written in words, and x is given as a number.
For example, let X = the...
17.8K
Randomized Experiments01:13

Randomized Experiments

9.1K
The randomization process involves assigning study participants randomly to experimental or control groups based on their probability of being equally assigned. Randomization is meant to eliminate selection bias and balance known and unknown confounding factors so that the control group is similar to the treatment group as much as possible. A computer program and a random number generator can be used to assign participants to groups in a way that minimizes bias.
Simple randomization
Simple...
9.1K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.2K
Overview
80.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coaxial Electrospray of Nanodrug-Loaded Porous Polylactic Acid/Poly(Ethylene Oxide) Core-Shell Microparticles for Intrapulmonary Drug Delivery.

Journal of biomedical materials research. Part A·2025
Same author

Electrosprayed Micro-to-Nanoscale Particles with Tunable Morphologies and Compositions for Pulmonary Drug Delivery Applications and Inhalation Therapies: A Review.

ACS biomaterials science & engineering·2025
Same author

Engineering polystyrene microtube-embedded composite hydrogels for tunable vascular morphogenesis.

Biomedical materials (Bristol, England)·2025
Same author

Cast-Molded Channelized Hydrogel Scaffolds With Stereolithography-Printed Templates.

Biotechnology and bioengineering·2025
Same author

Deep hybrid modeling of a HEK293 process: Combining long short-term memory networks with first principles equations.

Biotechnology and bioengineering·2024
Same author

Hybrid deep modeling of a CHO-K1 fed-batch process: combining first-principles with deep neural networks.

Frontiers in bioengineering and biotechnology·2023

Related Experiment Video

Updated: Feb 4, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.3K

Engineering the hard-soft tissue interface with random-to-aligned nanofiber scaffolds.

John Nowlin1, Mehzubh A Bismi1, Baptiste Delpech2

  • 1Department of Mechanical Engineering, Texas Tech University, Lubbock, Texas, USA.

Nanobiomedicine
|October 11, 2018
PubMed
Summary

This study created a gradient scaffold to regenerate the tendon-to-bone interface, mimicking natural enthesis. The scaffold successfully promoted tissue regeneration, offering a new solution for complex tissue repair.

Keywords:
Electrospinningenthesisheterogeneous nanofiber scaffoldtendontissue engineering

More Related Videos

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
06:14

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Published on: January 7, 2019

7.3K
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
08:35

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

Published on: April 8, 2011

16.1K

Related Experiment Videos

Last Updated: Feb 4, 2026

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
09:32

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

10.3K
Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
06:14

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Published on: January 7, 2019

7.3K
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
08:35

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

Published on: April 8, 2011

16.1K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tendon injuries often heal poorly, with high relapse rates due to scar tissue and surgical repair methods.
  • Regenerating the tendon/ligament-to-bone interface is crucial for successful graft integration after injury.

Purpose of the Study:

  • To recreate the native tendon-to-bone interface using a gradient scaffold fabricated via electrospinning.
  • To investigate the influence of scaffold microstructure on cell behavior and enthesis formation.

Main Methods:

  • Fabrication of a poly-ε-caprolactone nanofiber scaffold with a gradient transition from random to aligned patterns using electrospinning.
  • Co-culturing osteosarcoma and fibroblast cells on the respective random and aligned sections of the scaffold.
  • Assessing the impact of electrospinning parameters (polymer concentration, tip-to-collector distance, time) on scaffold microstructure.

Main Results:

  • A gradient scaffold successfully mimicked the native enthesis transition with a random-to-aligned cocultured tissue interface after 96 hours.
  • The scaffold's microstructure gradient significantly influenced cell morphology and tissue topology.
  • The developed scaffold promoted enthesis formation, indicating successful interfacial tissue regeneration.

Conclusions:

  • A heterogeneous nanofiber scaffold strategy can effectively regenerate interfacial tissues.
  • This approach offers a potential solution for mimicking transitional interfaces between different tissue types.
  • The platform can be further developed for creating complex multi-tissue systems.