Biochar amendment suppresses N₂ O emissions but has no impact on ¹⁵ N site preference in an anaerobic soil

Biochar amendments often decrease N₂ O gas production from soil, but the mechanisms and magnitudes are still not well characterized since N₂ O can be produced via several different microbial pathways. We evaluated the influence of biochar amendment on N₂ O emissions and N₂ O isotopic composition, including ¹⁵ N site preference (SP) under anaerobic conditions.

An agricultural soil was incubated with differing levels of biochar. Incubations were conducted under anaerobic conditions for 10 days with and without acetylene, which inhibits N₂ O reduction to N₂ . The N₂ O concentrations were measured every 2 days, the SPs were determined after 5 days of incubation, and the inorganic nitrogen concentrations were measured after the incubation.

The SP values with acetylene were consistent with N₂ O production by bacterial denitrification and those without acetylene were consistent with bacterial denitrification that included N₂ O reduction to N₂ . There was no effect of biochar on N₂ O production in the presence of acetylene between day 3 and day 10. However, in the absence of acetylene, soils incubated with 4% biochar produced less N₂ O than soils with no biochar addition. Different amounts of biochar amendment did not change the SP values.

Our study used N₂ O emission rates and SP values to understand biochar amendment mechanisms and demonstrated that biochar amendment reduces N₂ O emissions by stimulating the last step of denitrification. It also suggested a possible shift in N₂ O-reducing microbial taxa in 4% biochar samples.