Related Experiment Video
Updated: Feb 4, 2026

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Published on: January 11, 2017
The Response of Liposomes Modified with Lysosomal Membrane Proteins to the Targeting Ability Associated with
Abstract:
Liposomes were modified using two different methods. In Method 1, liposomes were modified by mixing whole lysosomal proteins, lipid, and cholesterol before preparation. For Method 2, the liposomes were modified by mixing whole lysosomal proteins after liposome preparation. Method 1-modified liposomes exhibited improved cell mortality compared to Method 2-modified liposomes. The modified liposomes were then evaluated for their antimicrobial activity against lysosomal enzymes, and Escherichia coli did not modify the liposome surface. The whole lysosomal membrane proteins extracted from the lysosomes in Saccharomyces cerevisiae were analyzed using two-dimensional electrophoresis to find specific proteins associated with antimicrobial activity and to construct recombinant S. cerevisiae proteins. Additionally, genes related to antimicrobial activity were identified, and the liposomes modified by lysosomal membrane proteins of recombinant S. cerevisiae tagged with green fluorescence proteins were prepared and overexpressed. The modified liposomes exhibited improved antimicrobial activity with an almost two-fold increase in the cell mortality rate, suggesting crucial roles as potential therapeutics.
Related Concept Videos
Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Introduction to Membrane Proteins
Lysosomes
Membrane Proteins
Target Cell Response to Hormones
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Lysosomal Hydrolases

