Related Experiment Video
Updated: Feb 4, 2026
07:52
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
20.5K
Cutaneous immunohistochemical staining pattern of p53β isoforms.
Christine J Ko1, Peggy Myung2, David J Leffell2
1Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut, USA christine.ko@yale.edu.
Journal of Clinical Pathology
|October 12, 2018
Summary
p53β isoforms are found in differentiated skin cells and hair follicles. Their expression patterns in squamous tumors suggest p53β may indicate keratinocyte differentiation.
Area of Science:
- Molecular biology
- Cancer research
- Dermatology
Background:
- The tumor suppressor protein p53, encoded by the TP53 gene, is crucial for genome integrity.
- TP53 is frequently mutated in various cancers, but the functional impact of these mutations is not fully understood.
- Multiple p53 isoforms exist, contributing to pathway complexity, yet their specific roles and differential expression are often unexamined.
Purpose of the Study:
- To investigate the expression patterns of p53β isoforms in skin tissues.
- To correlate p53β expression with mutational status in skin squamous proliferations.
Main Methods:
- Immunohistochemical staining for p53β isoforms in skin samples.
- Analysis of TP53 mutational status in a subset of squamous proliferations.
Main Results:
- p53β isoforms exhibit distinct cytoplasmic localization in the differentiated epidermis and hair follicles.
- Diffuse cytoplasmic expression of p53β was observed in well-differentiated squamous tumors harboring TP53 mutations affecting the tetramerization domain.
Conclusions:
- p53β isoforms demonstrate specific localization patterns in keratinocytes.
- The findings support the potential of p53β isoforms as biomarkers for keratinocyte differentiation in skin.
Related Concept Videos
01:06Fixed Action Patterns
17.7K
A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.
17.7K
01:26Patterns of Fever
3.9K
Before understanding the types and patterns of fever, it is essential to know its phases.
3.9K
01:29Oxygen Requirements and Growth Patterns
1.4K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
1.4K
01:13¹H NMR Signal Multiplicity: Splitting Patterns
6.9K
When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
6.9K
01:29Assessment of the Gastrointestinal System II: Health Perception Pattern
493
Assessing the gastrointestinal (GI) system is a complex process that begins with collecting subjective data. This data, collected through patient interviews, provides crucial insights into the patient's health history, perception patterns, and lifestyle habits, all contributing significantly to GI health.
Health Perception Patterns
Health perception patterns offer valuable insights into a patient's lifestyle habits and how they may impact their GI health. These patterns include:
Health Perception Patterns
Health perception patterns offer valuable insights into a patient's lifestyle habits and how they may impact their GI health. These patterns include:
493
01:24Simple Staining Technique
3.7K
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
3.7K