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Related Experiment Videos

Identifying HSV infected neurons after ocular inoculation.

T Margolis, B Togni, J LaVail

    Current Eye Research
    |January 1, 1987
    PubMed
    Summary

    This study shows that herpes simplex virus (HSV) actively replicates within neurons in the nervous system. Autoradiography reveals labeled neurons, indicating viral DNA synthesis in infected mice.

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    Area of Science:

    • Neurovirology
    • Infectious Diseases
    • Microscopy Techniques

    Background:

    • Herpes simplex virus (HSV) is a significant human pathogen.
    • Understanding HSV neurotropism and replication is crucial for developing effective treatments.
    • Current methods for studying viral replication in neural tissues have limitations.

    Purpose of the Study:

    • To develop and validate a novel autoradiographic technique for studying HSV replication in neural tissues.
    • To investigate the sites of HSV replication within the nervous system of infected mice.
    • To differentiate between neurons actively synthesizing HSV and those harboring virus from elsewhere.

    Main Methods:

    • Intracamercal inoculation of ICR mice with McKrae strain HSV.
    • Intraperitoneal injection of 3H-thymidine to label replicating DNA.
    • Autoradiography of embedded, sectioned eyes, trigeminal ganglia (TG), and superior cervical ganglia (SCG) after 3-4 days.
    • Light microscopy analysis of labeled neurons.

    Main Results:

    • Silver grain labeling was observed over neurons in the ipsilateral retina, TG, and SCG of HSV-infected mice.
    • No neuronal labeling was detected in the contralateral TG or SCG.
    • Labeled neurons are interpreted as cells actively replicating HSV, as mature neurons do not replicate their own DNA.

    Conclusions:

    • The developed autoradiographic technique provides excellent tissue preservation for studying HSV infection in the nervous system.
    • This method successfully identifies neurons with active HSV replication.
    • The technique can distinguish between intrinsic viral synthesis within neurons and virus spread from distant sites.

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