Related Experiment Video

Updated: Feb 4, 2026

Arthroscopic Management of Massive Irreparable Rotator Cuff Tears: Whole Rotator Cable Reconstruction Using Proximal Biceps Tendon Autograft
07:22

Arthroscopic Management of Massive Irreparable Rotator Cuff Tears: Whole Rotator Cable Reconstruction Using Proximal Biceps Tendon Autograft

Published on: June 6, 2025

655

Genes interconnecting AMPK and TREM-1 and associated microRNAs in rotator cuff tendon injury

Finosh G Thankam1, Chandra S Boosani1, Matthew F Dilisio1

  • 1Departments of Clinical & Translational Science and Orthopedic Surgery, Creighton University School of Medicine, Omaha, NE, 68178, USA.

Molecular and Cellular Biochemistry
|October 12, 2018
PubMed

Insights

Fatty infiltration and inflammation in rotator cuff tendon injuries (RCTI) are linked to specific microRNAs (miRNAs). Understanding these miRNAs may lead to new therapeutic strategies for healing RCTI.

Area of Science:

  • Biomedical Science
  • Molecular Biology
  • Orthopedics

Background:

  • Rotator cuff tendon injuries (RCTI) involve fatty infiltration and inflammation, complicating healing.
  • AMP-activated protein kinase (AMPK) and TREM-1 are upregulated in RCTI, but their epigenetic regulation by microRNAs (miRNAs) is unclear.
  • Understanding miRNA roles in RCTI pathophysiology could offer therapeutic avenues.

Purpose of the Study:

  • To investigate microRNAs (miRNAs) associated with fatty infiltration and inflammation in rotator cuff tendon injuries (RCTI).
  • To identify potential miRNA targets within the AMPK and TREM-1 signaling pathways in RCTI.

Main Methods:

  • Compared miRNAs in RCTI patients with (FI group) versus without (No-FI group) fatty infiltration and inflammation.
  • Utilized NetworkAnalyst to analyze gene networks involving AMPK (PRKAA1), TREM-1, HIF1α, HMGB1, and AGER.
  • Screened for miRNAs with fold change < -7.5 and ≥10 target genes.

Main Results:

  • Identified 13 key miRNAs and 216 target genes potentially involved in RCTI-related fatty infiltration and inflammation.
  • Established a network analysis connecting AMPK and TREM-1 pathways through specific miRNAs.

Conclusions:

  • Specific miRNAs are implicated in the fatty infiltration and inflammation observed in RCTI.
  • Further research into the biological activity of these identified miRNAs could facilitate the development of novel miRNA-based therapeutics for RCTI management.
Keywords:
AMPK signalingFatty infiltrationHMGB1InflammationRotator cuff tendon injuryTREM-1miRNA

More Related Videos

Development of a Rabbit Chronic-Like Rotator Cuff Injury Model for Study of Fibrosis and Muscular Fatty Degeneration05:20

Development of a Rabbit Chronic-Like Rotator Cuff Injury Model for Study of Fibrosis and Muscular Fatty Degeneration

Published on: March 31, 2023

1.8K
A Novel Arthroscopic Medial Knot-Tying Suture-Bridge Repair with Rip-Stop Technique for Rotator Cuff Tears06:41

A Novel Arthroscopic Medial Knot-Tying Suture-Bridge Repair with Rip-Stop Technique for Rotator Cuff Tears

Published on: January 13, 2026

97

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
Kinematic Equations for Rotation01:30

Kinematic Equations for Rotation

In mechanics, when one observes a rigid body in rotational motion with constant angular acceleration, it is possible to establish equations for its rotational kinematics. This process resembles how linear kinematics are dealt with in simpler motion studies.
For instance, imagine a point A on a rigid body engaged in circular motion. The translational velocity of this particular point can be calculated by taking the time derivatives of the displacement equation, which essentially measures the...
812
Rotation of Asymmetric Top01:11

Rotation of Asymmetric Top

By definition, a spherically symmetric body has the same moment of inertia about any axis passing through its center of mass. This situation changes if there is no spherical symmetry. Since most rigid bodies are not spherically symmetric, these require special treatment.
The relationship between the angular momentum of any rigid body and its angular velocity, both of which are vectors, involves the moment of inertia. The moment of inertia is a scalar quantity only for spherically symmetric...
1.5K
Rotation with Constant Angular Acceleration - I01:37

Rotation with Constant Angular Acceleration - I

If angular acceleration is constant, then we can simplify equations of rotational kinematics, similar to the equations of linear kinematics. This simplified set of equations can be used to describe many applications in physics and engineering where the angular acceleration of a system is constant.
Using our intuition, we can begin to see how rotational quantities such as angular displacement, angular velocity, angular acceleration, and time are related to one another. For example, if a flywheel...
8.4K
Rotation with Constant Angular Acceleration - II01:16

Rotation with Constant Angular Acceleration - II

Kinematics is the description of motion. The kinematics of rotational motion discusses the relationships between rotation angle, angular velocity, angular acceleration, and time. One can describe many things with great precision using kinematics, but kinematics does not consider causes. For example, a large angular acceleration describes a very rapid change in angular velocity without any consideration of its cause. Thus, rotational kinematics does not represent the laws of nature.
The first...
7.3K