Related Experiment Video
Updated: Feb 4, 2026
10:37
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
8.7K
Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine
Xuehui Yang1, Hongmei Chen2, Yan Chen2
1Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, China.
Summary
Dexmedetomidine alters circulating microRNAs (miRNAs) in patients, with twelve differentially expressed miRNAs identified. Specific miRNAs like hsa-miR-101-3p may be key targets for understanding dexmedetomidine
Area of Science:
- Biochemistry and Molecular Biology
- Pharmacology
- Genomics
Background:
- Circulating microRNAs (miRNAs) are potential biomarkers for cardiovascular and cerebrovascular diseases.
- Dexmedetomidine exhibits protective effects across various organs, but its impact on circulating miRNAs is not well understood.
Purpose of the Study:
- To investigate the differential expression of circulating miRNAs following dexmedetomidine administration.
- To predict target genes and associated pathways of the identified differentially expressed miRNAs.
Main Methods:
- High-throughput miRNA sequencing was employed to analyze circulating miRNA expression in three patients.
- Target gene prediction was performed using TargetScan 7.1 and miRDB v.5.
- Functional annotation and pathway enrichment analysis were conducted using Gene Ontology (GO) and KEGG.
Main Results:
- Twelve differentially expressed miRNAs were identified after dexmedetomidine administration.
- Five miRNAs were found to be upregulated, and seven were downregulated.
- Target genes and related biological pathways for these miRNAs were predicted and analyzed.
Conclusions:
- The identified differentially expressed miRNAs may play a role in the mechanism of action of dexmedetomidine.
- Specific miRNAs, including hsa-miR-101-3p, hsa-miR-150-5p, and hsa-miR-140-3p, represent novel targets for future research into dexmedetomidine's effects.
Related Concept Videos
02:27Predicting Molecular Geometry
45.8K
VSEPR Theory for Determination of Electron Pair Geometries
45.8K
01:15Receiver Operating Characteristic Plot
486
A ROC (Receiver Operating Characteristic) plot is a graphical tool used to assess the performance of a binary classification model by illustrating the trade-off between sensitivity (true positive rate) and specificity (false positive rate). By plotting sensitivity against 1 - specificity across various threshold settings, the ROC curve shows how well the model distinguishes between classes, with a curve closer to the top-left corner indicating a more accurate model. The area under the ROC curve...
486
01:21Coronary Circulation
7.3K
The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively.
Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...
Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...
7.3K
01:14Fetal Circulation
3.0K
Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta.
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
3.0K
01:03Prediction Intervals
3.4K
The interval estimate of any variable is known as the prediction interval. It helps decide if a point estimate is dependable.
However, the point estimate is most likely not the exact value of the population parameter, but close to it. After calculating point estimates, we construct interval estimates, called confidence intervals or prediction intervals. This prediction interval comprises a range of values unlike the point estimate and is a better predictor of the observed sample value, y.
However, the point estimate is most likely not the exact value of the population parameter, but close to it. After calculating point estimates, we construct interval estimates, called confidence intervals or prediction intervals. This prediction interval comprises a range of values unlike the point estimate and is a better predictor of the observed sample value, y.
3.4K
01:19Overview of Pulmonary Circulation
3.1K
The pulmonary circulation is a vital system in our body that acts as a bridge between the respiratory and cardiovascular systems. It serves as a transport network for deoxygenated blood from the heart to the lungs and then returns oxygen-rich blood back to the heart.
The process begins with the right ventricle of the heart pumping deoxygenated blood into the pulmonary trunk. This large vessel extends about 5 centimeters before splitting into the left and right pulmonary arteries. These arteries...
The process begins with the right ventricle of the heart pumping deoxygenated blood into the pulmonary trunk. This large vessel extends about 5 centimeters before splitting into the left and right pulmonary arteries. These arteries...
3.1K