[Effect of promoter duplication in the synthetic interferon gene on the level of its expression]

The expression of the artificial gene for the human leukocyte interferon (IFN) synthesized by the chemo-enzymic method has been studied in Escherichia coli cells. The genetic constructions in which the IFN gene transcription is carried out from A2 and B promoters of T7 phage or from E. coli lacZ UV5 gene promoter have been obtained, based on pEMB101 plasmid. In addition, the level of interferon production, depending on the promoter used, has been studied. It has been shown that the tandemly located promoters increase efficiency of the IFN gene expression.