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Updated: Feb 4, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
Simultaneous determination and risk assessment of fipronil and its metabolites in sugarcane, using GC-ECD and
Subrata Biswas1, Rahul Mondal2, Ayan Mukherjee2
1Department of Chemistry, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India.
Abstract:
A sensitive gas chromatographic method using a modified QuEChERS technique is reported for simultaneous determination, dissipation and risk assessment of fipronil and its metabolites in sugarcane and soil. Recoveries were 80.7-98.5% with precision within 1.4-16.5% estimated at the limits of detection (LOD) 0.0015-0.002 µg g-1 and limits of quantification (LOQ) 0.005 µg g-1. Fipronil dissipated with half-life (T1/2) of 2.8-4.3 days while for total fipronil it was 3.7-6.0 days following application of fipronil (5% SC) in sugarcane fields at recommended (100 g a.i. ha-1) and double the recommended (200 g a.i. ha-1) doses. Estimated pre-harvest intervals (PHI) for fipronil were 20.3-27.0 days in sugarcane plants, and for total fipronil the corresponding values were 28.2-37.8 days. No dietary risk was observed due to fipronil (RQd < 1) 5 days after application. Potential risk exists towards algae and soil macro-organism (RQs > 1), but for earthworms it was safe (RQs < 1).
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