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Updated: Feb 4, 2026

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
Published on: December 22, 2011
A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging
Wei Sheng1, Setare Tahmasebi Nick1, Elizabeth M Santos2
1Department of Chemistry, Michigan State University, E. Lansing, MI, 48824, USA.
Abstract:
FR-1V, a fluorene-based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF-2, a prototypic protein-dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV-light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no-wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence background.
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