Related Experiment Video
Updated: Feb 4, 2026

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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Published on: September 3, 2009
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HLA-DPB1*64:01N and DPB1*701:01 sequence extensions by single molecule real-time DNA sequencing
Charlotte A Cambridge1, Thomas R Turner1,2, Jashan P Abraham1
1Anthony Nolan Research Institute, Royal Free Hospital, London, UK.
HLA
|October 13, 2018
Summary
Pacific Biosciences
Area of Science:
- Immunogenetics
- Molecular Biology
Background:
- Human Leukocyte Antigen (HLA) genes are crucial for immune response.
- Accurate HLA typing is essential for transplantation and disease association studies.
Purpose of the Study:
- To confirm and extend the characterization of specific HLA-DPB1 alleles.
- To leverage advanced sequencing technology for high-resolution HLA typing.
Main Methods:
- Single Molecule, Real-Time (SMRT) sequencing by Pacific Biosciences.
- Applied to analyze and define HLA-DPB1*64:01N and HLA-DPB1*701:01 alleles.
Main Results:
- Successful confirmation of the existing HLA-DPB1*64:01N allele sequence.
- Extension and detailed characterization of the HLA-DPB1*701:01 allele.
Conclusions:
- SMRT sequencing provides a robust method for high-resolution HLA allele characterization.
- The findings contribute to the comprehensive understanding of HLA polymorphism.
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