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Imaging Glioma Progression by Intravital Microscopy
Fabio Stanchi1, Ken Matsumoto2, Holger Gerhardt2,3
1VIB-KU Leuven Center for Cancer Biology (CCB), Leuven, Belgium. Fabio.Stanchi@kuleuven.vib.be.
Methods in Molecular Biology (Clifton, N.J.)
|October 14, 2018
Summary
This study introduces a novel method for creating and tracking mouse brain gliomas using advanced microscopy. This technique allows for real-time observation of tumor growth and interactions, aiding treatment evaluation.
Area of Science:
- Neuroscience
- Oncology
- Biomedical Imaging
Background:
- Orthotopic glioma models are crucial for studying brain tumor progression.
- Non-invasive, longitudinal imaging of these tumors in vivo remains a challenge.
Purpose of the Study:
- To develop and validate a method for generating and chronically imaging orthotopic gliomas in mice.
- To enable multicolor 3D imaging of tumor progression and host interactions over time.
Main Methods:
- Surgical implantation of glioma cells into the mouse brain cortex.
- Cranial window implantation for chronic multi-photon laser scanning microscopy.
- Utilizing fluorescent proteins for multicolor 3D imaging of tumor and host cells.
Main Results:
- Successful generation of orthotopic gliomas in mice.
- Demonstration of chronic, multicolor 3D imaging of tumor progression.
- Visualization of tumor-host cell interactions in real-time.
Conclusions:
- The described method provides a powerful tool for studying glioma progression in vivo.
- This technique facilitates the evaluation of pharmacological treatments and basic research into tumor biology.
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