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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
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Updated: Feb 4, 2026

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"
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Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles.

Yulia Zhitnyuk1, Peter Gee2, Mandy S Y Lung2

  • 1Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia.

Biochemical and Biophysical Research Communications
|October 15, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a new mRNA delivery system using chimeric virus-like particles (VLPs). This novel system efficiently delivers mRNA to difficult-to-transfect cells, offering a transient gene delivery tool.

Keywords:
L7Ae RNA-binding proteinVLPsmRNA delivery

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Delivery

Background:

  • Messenger RNA (mRNA) delivery offers advantages over DNA delivery due to its transient nature and lack of genomic integration risk.
  • Current mRNA delivery methods are limited, particularly for challenging cell types, necessitating the development of new systems.
  • Efficient and transient gene delivery is crucial for various research and therapeutic applications.

Purpose of the Study:

  • To establish a novel and efficient mRNA delivery system.
  • To overcome limitations of existing delivery methods for hard-to-transfect cells.
  • To provide a transient gene delivery tool for mRNA of interest.

Main Methods:

  • Generation of a chimeric virus-like particle (VLP) by fusing Vesicular stomatitis virus protein G (VSV-G) with Archeoglobus fulgidus ribosomal protein L7Ae.
  • Utilizing the VSVG-L7Ae VLP system for mRNA delivery.
  • Assessing transduction efficacy in various cell lines, including human induced pluripotent stem cells (iPS cells) and monocytes.

Main Results:

  • The VSVG-L7Ae VLP system enabled efficient delivery of enhanced green fluorescent protein (EGFP) mRNA.
  • mRNA delivery was effective regardless of the presence of the BoxC/D motif in the mRNA sequence.
  • High transduction efficacy was observed in hard-to-transfect cell lines like iPS cells and monocytes.

Conclusions:

  • The developed VSVG-L7Ae VLP platform serves as an efficient system for mRNA delivery.
  • This VLP system demonstrates high potential for transient transgene delivery.
  • The platform offers a promising solution for delivering mRNA to challenging cell types in research and potential therapeutic settings.