A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis
Akio Aono1, Kinuyo Chikamatsu1, Hiroyuki Yamada1
1Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan anti-tuberculosis Association, Kiyose, Japan.
Abstract:
We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its potential application in phenotypic pyrazinamide susceptibility testing.
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