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Updated: Feb 4, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
Published on: May 3, 2015
Solvent directed selective and sensitive fluorescence detection of target ions using a coumarin-pyridine probe
Yiting Xie1, Wenjing Cheng, Bing Jin
1Jiangsu Key Laboratory of Pesticide Science, Department of Chemistry, College of Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. ybding@njau.edu.cn njzhangwh@126.com.
Abstract:
The development of fluorescent probes to selectively and sensitively detect more than one target analyte under a given condition is a challenging task due to the lack of designing strategies. In this paper, we report the utilisation of the solvatochromic properties of a coumarin-pyridine based probe L1 to detect Mg2+ and PPi, respectively, by using different solvents such as acetonitrile, DMF and DMF-HEPES buffered solutions. When acetonitrile was used as the solvent, L1 was weakly fluorescent and showed a selective and sensitive fluorescence "turn on" response towards Mg2+ with a detection limit of 86 nM, while in DMF and DMF-HEPES buffered solutions, L1 was brightly fluorescent and was able to form a non-fluorescent L1-Fe3+ complex. The obtained L1-Fe3+ complex was further determined to be a selective and sensitive fluorescent "turn on" probe for PPi with a detection limit of 94 nM.
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