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    Area of Science:

    • Biomedical optics
    • Ophthalmic imaging
    • Advanced imaging technologies

    Background:

    • Optical coherence tomography (OCT) is a key biomedical imaging tool, typically using near-infrared wavelengths.
    • Visible light (VIS) OCT shows promise for high-resolution retinal imaging.
    • Adaptive optics (AO) systems enhance imaging resolution but often require complex sensors.

    Purpose of the Study:

    • To develop and demonstrate a sensorless adaptive optics (SAO) multimodal imaging system.
    • To utilize visible light OCT (VIS-OCT) for high-resolution retinal imaging.
    • To integrate structural and fluorescence imaging capabilities.

    Main Methods:

    • Employed a broadband supercontinuum VIS source for VIS-OCT.
    • Implemented a sensorless adaptive optics (SAO) approach for aberration correction.
    • Utilized coherence-gated, depth-resolved VIS-OCT images for image guidance.
    • Acquired fluorescence emission data following structural imaging.

    Main Results:

    • Achieved high-resolution volumetric retinal structural imaging using VIS-OCT.
    • Demonstrated successful image-guided SAO aberration correction.
    • Enabled simultaneous high-resolution structural and fluorescence imaging of the retina.
    • VIS-OCT proved effective for detailed retinal visualization.

    Conclusions:

    • The developed SAO multimodal imaging system driven by VIS-OCT provides high-resolution retinal imaging.
    • Sensorless adaptive optics combined with VIS-OCT effectively corrects aberrations for enhanced imaging.
    • This approach advances capabilities for detailed retinal structural and functional (fluorescence) assessment.