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Updated: Feb 3, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
A ratiometric fluorescent BODIPY-based probe for rapid and highly sensitive detection of cysteine in human plasma
Nannan Wang1, Yue Wang, Jinhua Gao
1Key Laboratory for Special Functional Materials of the Ministry of Education, Henan University, Kaifeng, 475004, P. R. China. zhangjian861208@126.com zhaow@henu.edu.cn.
Abstract:
Biological thiols, especially low molecular weight thiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play a pivotal role in physiological and pathological systems. Thus, the detection of biothiols is highly important for early diagnosis of diseases and evaluation of disease progression. Herein, we developed a highly selective and sensitive ratiometric fluorescent 8-Cl BODIPY-based probe with high fluorescence quantum yields. The probe displayed a sensitive response to Cys and Hcy over other biothiols, which can be visualized colorimetrically and/or fluorescently. The probe was successfully applied to detect Cys in human plasma, demonstrating its great value for practical application in biological sample analysis.
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