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PEG-Plasma Hydrogels Increase Epithelialization Using a Human Ex Vivo Skin Model.

Randolph Stone1, John T Wall, Shanmugasundaram Natesan

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International Journal of Molecular Sciences
|October 17, 2018
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Summary

This study developed an ex vivo human skin model to test wound healing therapies. Polyethylene glycol-platelet-free plasma hydrogel accelerated wound epithelialization and showed promising results for future wound care research.

Keywords:
biomaterialsdiscarded skinepithelializationex vivokeratinocytewound closure

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Wound Healing Research

Background:

  • In vitro cell culture models for wound healing lack in vivo relevance.
  • Ex vivo models offer a more physiologically accurate environment for testing therapies.

Purpose of the Study:

  • To establish an ex vivo human skin model for evaluating wound healing treatments.
  • To assess the efficacy of collagen, PEG-fibrin, and PEG-PFP biomaterials in a wound healing context.

Main Methods:

  • Discarded human skin was used to create excisional wounds.
  • Wounds were treated with collagen, PEG-fibrin, or PEG-PFP hydrogels and cultured for 14 days.
  • Cytotoxicity, epithelialization, and histological markers (CK-10, α-SMA, WG) were analyzed.

Main Results:

  • Polyethylene glycol-platelet-free plasma (PEG-PFP) hydrogel promoted faster wound epithelialization compared to controls and other treatments.
  • A stratified epidermis (CK-10+) developed over PEG-PFP treated wounds.
  • Cells positive for wheat germ (WG) and alpha-smooth muscle actin (α-SMA) were observed within the PEG-PFP hydrogel.

Conclusions:

  • An ex vivo human skin model was successfully established for practical wound healing therapy screening.
  • PEG-PFP hydrogel demonstrates potential for enhancing wound healing processes.
  • This model provides a more translatable platform for preclinical wound care research.