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Updated: Feb 3, 2026

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
Published on: November 12, 2017
Porcine RPE/Choroidal Explant Cultures
Alexa Klettner1, Yoko Miura2,3
1Department of Ophthalmology, University Medical Center, University of Kiel, Kiel, Germany. AlexaKarina.Klettner@uksh.de.
Abstract:
The cultivation of retinal pigment epithelium (RPE)-choroid explants gives the opportunity to study the RPE and Bruch's membrane in its natural environment. Porcine eyes are easily available and an excellent model for human RPE. Explants are prepared less than 4 h postmortem from cooled eyes and are transferred in fixation rings. The tissues held between rings are cultured in a perfusion organ culture system for up to a week. Viability of the explants can be investigated by calcein staining.
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