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Published on: June 7, 2024
Detection and Quantification of the Bicoid Concentration Gradient in Drosophila Embryos
Feng He1,2,3, Honggang Wu4,5, David Cheung6,7
1Division of Medical Genetics and Genomics, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Abstract:
We describe methods for detecting and quantifying the concentration gradient of the morphogenetic protein Bicoid through fluorescent immunostaining in fixed Drosophila embryos. We introduce image-processing steps using MATLAB functions, and discuss how the measured signal intensities can be analyzed to extract quantitative information. The described procedures permit robust detection of the endogenous Bicoid concentration gradient at a cellular resolution.
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