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Updated: Feb 3, 2026

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo
Published on: July 9, 2019
LiveFly: A Toolbox for the Analysis of Transcription Dynamics in Live Drosophila Embryos
Huy Tran1,2, Carmina Angelica Perez-Romero2,3, Teresa Ferraro1,4
1Ecole Normale Supérieure, PSL Research University, CNRS, Sorbonne Université, Laboratoire de Physique Théorique, Paris, France.
Abstract:
We present the LiveFly toolbox for quantitative analysis of transcription dynamics in live Drosophila embryos. The toolbox allows users to process two-color 3D confocal movies acquired using nuclei-labeling and the fluorescent RNA-tagging system described in the previous chapter and export the nuclei's position as a function of time, their lineages and the intensity traces of the active loci. The toolbox, which is tailored for the context of Drosophila early development, is semiautomatic, and requires minimal user intervention. It also includes a tool to combine data from multiple movies and visualize several features of the intensity traces and the expression pattern.
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