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Xian Zhang1,2, Zuohuan Wang3, Yun Fang4

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A new antibody microarray immunoassay enables simultaneous detection of four key mycotoxins in corn. This sensitive and accurate method offers a reliable tool for food safety testing.

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biotin-streptavidinmicroarraymycotoxinsquantification

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Immunotechnology

Background:

  • Mycotoxins pose significant risks to human and animal health.
  • Accurate and simultaneous detection of multiple mycotoxins is crucial for food safety.
  • Existing methods can be time-consuming or lack multiplexing capabilities.

Purpose of the Study:

  • To develop and validate a prototype antibody microarray immunoassay.
  • To enable simultaneous quantitative detection of four major mycotoxins in corn.
  • To enhance assay sensitivity and reliability for mycotoxin analysis.

Main Methods:

  • Development of a nitrocellulose membrane-based antibody microarray.
  • Immobilization of monoclonal antibodies specific to aflatoxin B₁, ochratoxin A, zearalenone, and fumonisin B₁.
  • Utilized biotinylation, biotin-streptavidin system, and enhanced chemiluminescence (ECL) for signal amplification.

Main Results:

  • Achieved high goodness of fit (R² > 0.98) for calibration curves.
  • Demonstrated low detection limits (ng/mL) and limits of detection (LOD in μg/kg) for all four mycotoxins.
  • Reported high recovery rates (79.2%–113.4%) and low coefficients of variation (<10%).
  • Results showed good agreement with liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Conclusions:

  • The developed antibody microarray immunoassay is sensitive, specific, and accurate for simultaneous mycotoxin detection in corn.
  • This assay provides a valuable tool for rapid and reliable food safety monitoring.
  • The platform is adaptable for detecting other mycotoxins and low-weight hazardous analytes.