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Utilization of Apatinib-Loaded Nanoparticles for the Treatment of Ocular Neovascularization
Kathleen Halasz1, Shannon J Kelly1, Muhammad Tajwar Iqbal1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, Tampa, FL 33612, United States.
Background:
The current treatment of ocular neovascularization requires frequent intravitreal injections of anti-vascular endothelial growth factor (VEGF) agents that cause severe side effects.
Objective:
The purpose of this study is to prepare and characterize a novel nanoscale delivery system of apatinib for ocular neovascularization.
Methods:
The optimized formulation showed a particle size of 135.04 nm, polydispersity index (PDI) of 0.28 ± 0.07, encapsulation efficiency (EE) of 65.92%, zeta potential (ZP) of -23.70 ± 8.69 mV, and pH of 6.49 ± 0.20. In vitro release was carried out to demonstrate a 3.13-fold increase in the sustainability of apatinib-loaded nanoparticles versus free apatinib solution.
Result:
Cell viability and VEGFA and VEGFR2 expression were analyzed in animal retinal pigment epithelial (ARPE-19) cells.
Conclusion:
The results confirmed the hypothesis that apatinib nanoparticles decreased toxicity (1.36 ± 0.74 fold) and efficient VEGF inhibition (3.51 ± 0.02 fold) via VEGFR2 mediation.
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